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Analysis of Orphenadrine Citrate in Various Chiral Stationary Phases: A Comparative Study

[ Vol. 7 , Issue. 2 ]

Author(s):

Prachi Raikar*, Bannimath M. Gurupadayya, Sripuram Subramanyam and Gunnam Srinivasu   Pages 91 - 100 ( 10 )

Abstract:


Background: Polysaccharide based chiral stationary phases (CSPs) were used to perform enantiomeric separation of Orphenadrine Citrate by Ultra-Fast Liquid Chromatography (UFLC) technique. Trials were conducted using the polar mode, reverse phase mode and normal phase mode. Amylose and Cellulose-based CSPs were used for the same.

Materials and Methods: Eight Amylose-based CSPs and four Cellulose-based CSPs were used in the reverse phase mode. Five Amylose-based CSPs and two Cellulose-based CSPs were used in polar mode. The only Cellulose-based CSP used in the normal phase mode could effectively separate Orphenadrine Citrate enantiomers with a good resolution.

Results and Discussion: Successful enantioseparation was obtained using Chiralcel OD-H containing Cellulose tris (3, 5- dimethylphenylcarbamate) as a chiral selector and n-hexane: Ethanol: Diethylamine (95: 05: 0.1, v/v/v) as the mobile phase. The developed method was validated in accordance with ICH guidelines (Q2R1).

Conclusion: The proposed objectives were successfully accomplished as the developed method could effectively resolve Orphenadrine Citrate enantiomers.

Keywords:

Chiral stationary phase, enantioseparation, orphenadrine citrate, ultra-fast liquid chromatography, amylose-based CSPs, cellulose-based CSPs.

Affiliation:

Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru- Karnataka, 570015, Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru- Karnataka, 570015, Department of Pharmaceutical Analysis, Daicel Chiral Technologies, Hyderabad-500101, Telangana, Department of Pharmaceutical Analysis, Daicel Chiral Technologies, Hyderabad-500101, Telangana



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